Living single cell sampling interface: Cell release and viability test

摘要

Cell analysis method is widely used in general biology and medicine. Recently, single cell analysis is highly required due to the cell heterogeneity. Currently, many methods were developed for the single cell analysis. However, there is a challenge in sampling from singe cell. In particular, sampling by maintaining the cell viability is highly demanded. For this purpose, we developed a living single cell sampling interface utilizing microanofluidic technologies. Single cell was connected to the nanochannel by lipid fusion method, and fL sampling was achieved. In this presentation, we will show the cell viability after the sampling. Monitoring method of lipid fusion without fluorescence staining was developed. Cell release could be realized by nanofluidic control, and cell showed the viability for more than 12 hours. Concept of living single cell interface was verified. This method will be quite useful for proteomics/metabolomics at single cell level. Due to the cell viability after sampling, this method allows to monitor the cellular dynamic process and also have large impact on cell biology, tissue engineering, cell therapy, etc.