The Effect of Acute Allergen Exposure on Adiponectin Levels in Serum and Adipose Tissue

摘要

Background: Epidemiological studies indicated that allergen exposure can be linked to higher risk of cardiovascular diseases (CVD). Compared with health population, patients with CVD has lower serum adiponectin levels which play a protection role for CVD. However, remains less understood how the allergen exposure to impact on adipose tissue and later the adiponectin levels. Aims: The objective of this study is to investigate whether acute exposure to OVA (Ovalbumin) allergen can affect adiponectin levels of serum and adipose tissue which play a protective role in diabetes or cardiovascular diseases. Methods: An acute intranasal challenge model with OVA (Ovalbumin) using male C57BL/6 mice was performed at dose of 6.25,12.5, 25, 50 and 100 μg, and control mice was challenged with PBS (Phosphate Buffered Saline). BALF (Bronchoalveolar lavage fluid), serum and adipose tissues were collected 24 hours after the last intranasal challenge. IL-5 of BALF, adiponectin, total IgE and OVA-specific IgE of serum were analyzed by ELISA (Enzyme-linked immunosorbent assay). Real-time PCR was used to analyze the expressions of IL-6 and TNF-α in gonadal and inguinal adipose tissues. Results: Results showed that acute OVA exposure did not only cause airway inflammation in study mice, but also decrease adiponectin levels in serum with a dose-response effect. When examining the gonadal adipose tissues, there was no significantly difference of adiponectin mRNA in OVA challenged mice compared to those PBS challenged, but lower inguinal adiponectin mRNA expression was found compared to those PBS-challenged. All adipose tissues of OVA challenged mice, including gonadal and inguinal, have higher IL-6 and TNF-α expression. Conclusions: Our data indicate that acute OVA exposures appear to cause inflammation in adipose tissues, and affect the adiponectin levels in serum and adipose tissues. Allergen exposure is therefore considered a potential risk factor for presenting diabetes or cardiovascular diseases.