The 3,6S-chitosan-modified surface showed specific adsorption of lysozyme and then had the high hydrolytic activity toward bacterial cell walls, which ensured the surface's high antibacterial activity. Moreover, the regeneration of the 3,6S-chitosan-grafted surface by a simple high-salinity treatment allowed it to regain its ability to specifically bind lysozyme and kill E. coli cells. This research extended sulfated chitosan's range of antibacterial properties and developed a new strategy for preparing recyclable antibacterial surfaces by using 3,6S-chitosan.
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