Assessment of Antioxidant Copolymers for Improved ROS Scavenging in Post Traumatic Osteoarthritis

摘要

These studies demonstrate the promising potential of DMA-co-TEMPO polymers as an antioxidant therapy for inflammation-driven PTOA. Hydrophilic modification and a balance of ROS scavenging in DMA-co-TEMPO polymers are necessary to provide bioactive efficacy in TEMPO-based PTOA delivery systems. Our data highlights the importance of compositional tuning, with increased water solubility driving cellular uptake while a more balanced ratio (60:40 DMA-co-TEMPO) optimized antioxidant potential. This random copolymer significantly reduced ROS and inflammatory cell presence in an in vivo model of inflammation. The in vivo results indicate that 60:40 DMA-co-TEMPO is a promising candidate as a PTOA therapy. Future studies will focus on the measurement of superoxide-specific scavenging by polymers in cell-free and activated immune cell-based assays.