Study Of The Correlations Between Direct Electron Transfer Rate Constants And The Effectiveness Of Cancer Inhibitors Using Nanobiomimetic Sensors

摘要

We report a new systematic approach to study Direct Electron Transfer (DET) rate constants between an electrode surface and an active biomimetic receptor site using four types of gold nanobiomimetic sensor chips. The DET results were obtained under antibody-free, label-free and analyte-free conditions in cell culture media using a cyclic voltammetry (CV) method. Sensor 1 has 20 nm nanopores, Sensor 2 has 40 nm nanopores, Sensor 3 has nano islands and Sensor 4 has a flat nano bridge with nanopores. The DET k_s results are sensor 4＞sensor 3＞sensorl＞sensor 2. Anti-cancer compounds l-Methyl-2-piperidinemethanol (MPPDM), Flavopiridol hydrochloride hydrate (FLAPHH) and o-nitrophenyl acetate (o-NPA) were used for evaluation of brain cancer cells of SNB-19 by a Chronoamperometric (CA) method and a CV method. Results shown the MPPDM and o-NPA completely blocked cancer signaling with sensor 4 at 5 cancer cells/mL, while sensor 3 was unable to detect at this level. Cancer detection rate is 97 ± 6% and 90 ± 4% for a CV and a double step chronopotentiometry (DSCPO) method, respectively at 50 cell/mL using cancer spiked NIST human standard sera SRM 965A against that of the samples in the media. Our preliminary results revealed the higher DET values may have a positive correlation to the effectiveness of the anti cancer compounds.