SUCROSE PROCESSING UPGRADE CARRIED OUT IN A CONTINUOUS MEMBRANE REACTOR USING INVERTASE, GLUCOSE OXIDASE AND CATALASE

摘要

This work deals with the use of the following enzymes, invertase (I), glucose oxidase (GO) and catalase, in order to convert sucrose into fructose and gluconic acid by using a 10mL-membrane reactor (MR) coupled to a 100kDa-ultrafiltration membrane. It was observed that the enzymes presented dissimilarity regarding their respective activity and thermal stability. The conversion was carried out through a two-step continuous process – invertase hydrolyzed sucrose and GO oxidized glucose. A 90% overall conversion in reference to the oxidation of glucose to gluconic acid was attained.