Simulation study of the effect of Mitragyna speciosa on hybrid current in rat hippocampus CA3 pyramidal neuron

摘要

We have simulated the hybrid current using Neurons in Action software to observe the hypothetical effect of Mitragyna speciosa in the model rat hippocampus CA3 pyramidal neuron at different Calcium ion conductance and at different temperature. Previous studies using the patch clamp technique showed that Mitragyna speciosa or Ketum could block long lasting Calcium channel (L-type Ca channel) currents in N1E-115 neuroblastoma cells. There is currently no such study of the effects of ketum on pyramidal neurons using the patch clamp technique. Our team is interested to study the ability of Ketum alakaloid to block L-type Ca channel in a rat's brain. The results of the simulation study gave us valuable information and guidelines for our future study to replicate the Ca channel blocking nature of the alkaloid of the Mitragyna speciosa.