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High-throughput automated system for statistical biosensing employing microcantilever arrays

机译:高通量自动化系统,采用微悬臂阵列进行统计生物传感

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In this paper we present a completely new and fully automated system for parallel microcantilever-based biosensing. Our platform is able to monitor simultaneously the change of resonance frequency (dynamic mode), of deflection (static mode), and of surface roughness of hundreds of cantilevers in a very short time over multiple biochemical reactions. We have proven that our system is capable to measure 900 independent microsensors in less than a second. Here, we report statistical biosensing results performed over a haptens-antibody assay, where complete characterization of the biochemical binding on the cantilever surfaces is obtained with higher accuracy than standard optical lever-based setups.
机译:在本文中,我们提出了一个全新的,基于微悬臂梁并行生物传感的全自动系统。我们的平台能够在很短的时间内通过多个生化反应同时监视共振频率(动态模式),挠度(静态模式)和数百个悬臂的表面粗糙度的变化。我们已经证明,我们的系统能够在一秒钟内测量900个独立的微传感器。在这里,我们报告了通过半抗原抗体测定法执行的统计生物传感结果,其中,与基于标准光杠杆的设置相比,以更高的准确度获得了悬臂表面上生化结合的完整表征。

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