An Integrated Strategy for Functional Analysis in 16S rRNA Gene-based Research of Microbial Communities by Gene Ontology

摘要

Tbe environment and human serve as elaborate bosts to microbes, including a diversity of commensal and pathogenic bacteria that contribute to both health and disease. 16S rRNA genes are used for community profiling, or determining the abundance of each kind of microbe. The aim of our study is to analyze the similarity among microbial communities on the functional state, after assigned 16S rRNA sequences from all microbial communities to species. It's an important addihon to the species-level relationship between two compared communities, and can quantify their differences in function. To accomplish this aim, we downloaded all functional annotation data of microbiota from related datasets. lt's developed to identify the functional distribution and the significantly enriched functional categories of microbial communities. Exploration of the function relationship between two sets of species assemblages will be a key result of microbiome studies and may provide new insights into assembly of a wide range of ecosystems.