Effect of Particle Size on Biodistribution of Liver Targeted Doxorubicin Loaded Nanoparticles in Rats

摘要

The present study discusses the effect ofparticle size on biodistribution of doxorubicinnanoparticles with and without pullulan, anasialoglycoprotein receptor ligand for targeting to theliver. Doxorubicin nanoparticles of two different sizesviz. 114.9±12.3 nm and 353.4±1.06 nm wereprepared by nanoprecipitation technique using poly(methyl vinyl ether co-maleic anhydride) (GantrezAN-119) as polymer. Nanoparticles wereradiolabelled with 99mTc. Biodistribution studieswere carried out in rats and were monitored bygamma scintigraphy. At the end of 5 hrs rats weresacrificed, various organs viz. Heart, lung, liver,spleen and kidney were isolated and radioactivitywas measured. The radioactivity (cpm) in eachorgan was determined under gamma. High uptake inliver of nanoparticles with pullulan (353.4±1.06nm) issuggestive of receptor mediated targeting to theliver. Nanoparticles with pullulan physicallyincorporated during nanoparticles preparationrepresent a good strategy for design of liver targeteddrug delivery systems.