A new NIRS method for high throughput analysis of oleic, linoleic and linolenic acid content of single seeds in oilseed rape

摘要

A high-throughput Near-Infrared Reflectance Spectroscopy (NIRS) method using an automated sample presentation unit for single seeds of oilseed rape and a new kind of spectrometer equipped with a photodiode array detector was developed. First analyses have been accomplished with a throughput of about 500 seeds per hour. Seeds from segregating F2 populations of different origin were analysed by NIRS and gas liquid chromatography (GLC). Calibration equations were developed and validated. The coefficients of determination in cross validation were 0.81 for oleic acid, 0.76 for linoleic acid and 0.57 for linolenic acid. The ratio standard deviation/standard error in cross validation (SD/SECV) ranged from 2.4 for oleic acid over 2.1 for linoleic acid to 1.7 for linolenic acid. Using four different external validation sets, the coefficients of determination in external validation varied between 0.44 and 0.88 for oleic acid, between 0.31 and 0.72 for linoleic acid and between 0.11 to 0.33 for linolenic acid.The results show that the new high-throughput method can be applied to predict the oleic and linoleic acid content of single rapeseeds. However, the calibration seed sample set need to be extended by more seeds derived from different crosses and environments before robust results can be expected in routihe analysis.