A molecular genetic linkage map in recombinant inbred lines of Brassica napus L. using SSR and SRAP markers

摘要

Brassica nupus L. (2n = 38, AACC) Recombination Inbred Lines (RILs) population were derived from a cross between Zhongyou 821 which was brown seed coat and GH 06 which was yellow seed coat. They have been recombined self-inbred by single seed descent method for seven years. 441 SSR primer pairs and 4096 SRAP primer combinations were used to screen for polymorphisms between black-seeded and yellow-seeded parents. 2536 of 4096 SRAP primer combinations did not yield distinct amplification bands. 92 SSR primer pairs (20.86%) and 260 SRAP primer combinations (16.67%) yielded 106 and 480 unambiguous polymorphic bands respectively. 423 loci (74 for SSR and 349 for SRAP) were mapped on 23 linkage groups ranging from 39 cM to 140 cM with an average length of 76.96 cM containing 3 to 82 markers in each linkage group by JoinMAP 3.0.. The map covered a total of 1770 cM with the average distance between two adjacent markers was 4.18 cM. In order to gain a molecular genetic map which can cover the entire genome, other molecular marker methods are required, for example AFLP and RFLP. The map has very important use in locating some Brassica napus L. quantity trait locus ( QTL ) and molecular marker assist breeding in the future.