Analysis of glucosinolate components of different DH populations in Brassica napus

摘要

Glucosinolate components of 4 DH populations from different donors derived from different breeding programs were investigated by high performance liquid chromatography (HPLC). Compared with the donors, the segregation oftotal glucosinolate and its components in DH progeny were investigated. The variations of main glucosinolate components were different among 4 populations. And the variances of total glucosinolate concentration in the DH lines derived from the Fls with low glucosinolates parents were the most remarkable. All the DH populations were also used to analyze the partial correlations among different glucosinolate components. Significant positive correlation (P＜0.01) was found between progoitrin and gluconapin as well as between 4-hydroxyglucobrassicin and gluconasturtiin, respectively. It indicated that there were common precursors or intermediates in their synthesizing pathway. Genotypes with high glucosinolates were more readily to form embryos.However, in the 4 DH populations, progeny with lower glucosinolate concentration still could be selected, which indicated microspore culture was useful to low-glucosinolate breeding while suitable population size should be considered.