Application of DNA microarray analysis for identification of lager yeast strains and detection of mutation in lager yeast

摘要

We developed DNA microarray which contained all genes and inter-genic sequences of lager brewing yeast and was expected to detect slight genomic differences among various yeast strains. Genomic hybridization of yeast genomes using this microarray revealed that genomic differences among lager strains occurred not only in intra-/ inter-genic sequences but also in numbers of chromosomes which could remarkably influence their fermentation performances. Furthermore, it was found that this type of difference was possibly promoted in lager yeast by oxygen stress. This microarray could discriminate lager strains and detect mutations which would be important to control beer quality.