primary Rat Hepatocyte Culture in a Packed Bed Reactor with Fibra-Cel Carriers

摘要

Major metabolic functions of rat hepatocytes cultured in a packed-bed bioreactor (NBS Spinner Basket) for more than 90 days were investigated. Fibra-Cel, a three dimensional polyester fiber carrier was used as a growth support material. The 500 mL working volume reactor contained a 100 mL fixed-bed basket which was loaded with 10 grams of Fibra-Cel disks. Serum containing Williams E media was used. A four-gas system for pH and DO control and a re-circulating perfusion strategy were used in the process.