An improved analytical method for the determination of perfluorinated compounds in whole blood using acetonitrile and solid phase extraction methods and separation of taurodeoxycholic acid from perfluorooctane sulfonate using an ion exchange column

摘要

A method using acetonitrile and OASIS WAX(R) solid phase extraction (SPE) cartridges for the analysis of perfluorinated compounds (perfluoroalkyl sulfonates: C4, C6, C8, C10; perfluorooctanesulfonamide; N-ethyl perfluorooctanesulfonamide; N-ethyl perfluorooctanesulfonamidoacetate; perfluorocarboxylates: C4-C14; fluorotelomer carboxylate (7:3, 8:2)) in whole blood was developed. Matrix recoveries of the developed methods ranged from 70 to 120%. Separation of target compounds in two HPLC columns (ion exchange JJ50-2D and C18 Betasil columns) was examined. Separation of possible inferences such as taurodeoxycholic acid (TDC) from PFOS was accomplished using an ion exchange JJ50-2D column, and this separation was validated using whole blood from different animals.