Impact of a-naphthalene Acetic Ccid on Peony Embryo Culture

摘要

Using the mature embryo of the tree peony cultivar viz. "Fengdan " as the explants, the effects of anaphthalene acetic acid (NAA) and the combination of NAA and 2,4-D on the culture of the tree peony mature embryo were primarily studied. The suitable NAA concentration and combination of NAA and 2,4-D in the callus induction medium of the mature embryo were defined. The results showed that, the optimization concentration of single NAA was 6.0-8.0 mg/1 (the optimization concentration was 7.0 mg/1 and the highest frequency of callus induction was 71.50%) and the optimization combination was NAA 1.2 mg/1 + 2,4-D 1.0 mg/1 (the frequency of callus induction was 74.32%) in the callus induction medium of the peony mature embryos. The results indicated that too, there was inhibiting effect of high concentration NAA (>1.0 mg/1) on the germination of mature embryos and the morphogenesis. This study has provided a theoretical basis for improving the tissue culture efficiency, establishing the simple and efficient in vitro clonal propagation system, genetic transformation and molecular plant breeding in the tree peony.