Crystal Structure of Basic Phospholipase A2 from Agkistrodon Halys Pallas Crystal

摘要

Phospholipase A2 (PLA2, EC 3.1.1.4) specifically catalyzes the hydrolysis of the C-2 ester bond of 3-sn-phosphoglycerides to produce lysophosphatidylcholine and fatty acids in a calcium-dependent reaction. In this communication, we describe the structures of the three crystal forms of the low molecular mass (14KD) basic phospholipase A2 (BPLA2) isolated from the venom of Agkistrodon halys Pallas. BPLA2 is a strong hemolytic toxin and one of the few PLA2 capable of hydrolyzing the phospholipids of E. coli membranes in the presence of a bactericidal /permeability-increasing protein (BPI) of neutrophils.