A Study of Anti-Lipid Peroxidation and Injury Protection Effects of Picroside Ⅱ on Hepatic Cellular Mitochondria

摘要

[Purpose] To study anti-lipid peroxidation and injury protection effects on hepatic cellular mitochondria in mice with liver damage. [Methods] Three animal models, with liver damage induced by carbon tetrachloride (CCl_4:0.1ml/10g, ip), D-galactosamine (D-GalN: 500 mg/kg, ip) and acetaminophen (AP: 0.15g/kg, ip ) respectively, were administered Picroside Ⅱ at various levels of concentration (5, 10, 20 mg/kg, ig). The continuously monitoring method, recommended by International Clinical Chemistry League, was used to analyze serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), while Marland method was used to detect the activitiy of manganese-superoxide dismutase (SOD) in liver mitochondria. TBA colorimetry was employed to determine the content of malonic aldehyde (MDA) in liver tissue. The activity of glutathioneperoxidase (GSH-Px) was evaluated by DTNB method and the protein level in liver tissue was detected by Lowry method. Meanwhile, protective effects of picroside Ⅱ on the activity of ATPase and swollen extent of mitochondria in AP damaged hepatocytes were also evaluated. RESULTS: Picroside Ⅱ showed significantly preventive effects on liver toxicity in the three models of liver damage. It decreased the high levels of ALT and AST in serum induced by the administration of CCl_4, D-GalN and AP, remarkably reduced livercellular damages, and appeared to be even more potent than the positive control drug of biphenyl dimethyl dicarboxylate pilules (DDB). In groups treated with different doses of Picroside Ⅱ, compared to the model group, the content of MDA in serum decreased evidently, whereas the content of SOD and GSH-Px increased in a dose-dependent manner, and the difference was statistically significant. Further, the results of the AP model showed that picroside Ⅱ could inhibit AP-induced liver toxicity in mice, enhance the activity of ATPase, improve the swell extent of mitochondria, and help maintain a normal balance of energy metabolism.[Conclusion] Picroside Ⅱ can protect hepatocyte injuries induced by CCl_4, D-GalN and AP. This protection effect may be achieved through the mechanism that Picroside Ⅱ can help scavenge free radicals, protect normal constructions of mitochondria membrane and enhance the activitiy of ATPase in mitochondria, resulting in modulating the balance of liver energy metabolism. A Study of Anti-Lipid Peroxidation and Injury Protection Effects of Picroside Ⅱ on Hepatic Cellular Mitochondria.