Selective Excitation Light Fluorescence (SELF) Imaging

摘要

Fluorescence imaging is a potential candidate for tissue diagnostics in a wide variety of clinical situations. In order tornextract diagnostic information using fluorescence, different approaches may be used. Typically, fluorescence imaging isrnperformed by illuminating the sample at a single excitation wavelength and detecting the emissions at one or morernwavelengths.rnWe have built a prototype system for a new fluorescence imaging technique denoted Selective Excitation LightrnFluorescence (SELF) Imaging. In this technique, the sample is illuminated with multiple excitation wavelengths, and onernor more emitted wavelength images are detected. By using a multitude of illumination wavelengths or a weighted sum ofrnillumination wavelengths, SELF imaging can highlight differences in the excitation spectra of fluorophores in thernsample.rnSome potential advantages of this imaging technique are: detection of multiple labeled objects in microscopy using onlyrna single filter cube, increasing the number of simultaneous labels which can be used on a single slide as labels arernseparated by their absorption spectra not just their emission spectra, detection of different components of tissue based onrndifferent excitation spectra, etc.