Effects of over-expressing of rice glycogenin-like protein(OsGGT1)on tolerance to complete submergence and drought stress

摘要

Glycogenin glucosyltransferases or glycogenins(GGTs)are members of glycosyltransferase family 8.In most organisms other than plants,GGT is involved in initiation of the biosynthesis of glycogen,an important energy storage carbohydrate.In higher plants genomes,proteins with similar sequences and conserved domains to glycogenins were found and some of these proteins have been shown to be upregulated by environmental stresses.In this study we have cloned the full sequence of the rice OsGGT1 gene,and evaluated the effects of its overexpression on starch contents,and on the tolerance to complete submergence and drought stress.The transgenic plants were generated by Agrobacterium-mediated gene transfer of the rice cultivar ‘Nipponbare’.Integration of the transgene was confirmed by genome PCR,while its expression was confirmed by northern and western blotting analyses.