In vivo lymph dynamic monitoring using speckle-correlation technique and light microscopy

摘要

In this work we described the new modification of experimental setup designed on the basis of transmission microscopy and high-resolution speckle-correlation technique. This combined technique provides the simultaneous speckle and video registration of lymph dynamics that allows one to calibrate the speckle-correlation velocity sensor and to determine an absolute flow velocity and its direction. As a result many parameters of lymph dynamic were measured quickly, conveniently and simultaneously and a new data about the lymph flow velocity and other functions of microcirculation were received. The results of the experimental study of lymph microcirculation in small intestine mesentery of rat in vivo are presented.