Suitable microscope objectives for multiphoton digital imaging

摘要

The proper choice of an objective lens can have a profound effect on multiphoton microscopy. Multiphoton requires that the objective have adequate transmission in the IR range. This technology has a requisite to image the specimen near the slide surface next to the cover glass and to of 200 microns or more. Fluor and Plan Apochromat objectives. All lenses suffer from aberrations, which can prevent imaging an exact replica of the object Most objectives could be used for multiphoton imaging; however lenses should be selected from Plan Fluors, Plan Apochromats, and Super (S) Fluors. Highly corrected water immersion objectives (WI) permit deep penetration within aqueous environments and living tissues, beyond that of oil immersion objectives. Spectral Transmission data is presented for Nikon CFI60 various objective lenses with high NAs, long working distances, and maximum aberration correction.