Time-resolved evanescent wave-induced fluorescence studies of macromolecular adsorption

摘要

Evanescent Wave (EW) fluorescence spectroscopic techniques are exploited to obtain information about the conformational state of macromolecules within close proximity of a solid/liquid interfacial region. Specifically, time-resolved evanescent wave-induced fluorescence techniques have been applied to the study of the adsorption of polymers and biomolecules to silica surfaces. We have extended these EW measurements using polarized excitation and emission detection to probe molecular motion and conformational change in the microenvironment of the interfacial region. We report on the observation of complex time-dependent fluorescence anisotropy data and the interpretation of these data in terms of in- and out-of-plane rotational motion. The macromolecular-interfacial systems investigated by this evanescent wave approach included polymer film dynamics and adsorbed protein rearrangement upon adsorption.