Crystallization process for highly purified succinic acid from fermentation broth

摘要

Succinic acid is a four-carbon dicarboxylic acid produced as one of the fermentation products of anaerobic metabolism. Based on the complete genome sequence of a capnophilic succinic acid-producing rumen bacterium, Mannheimia succiniciproducens, gene knockout studies were carried out to understand its anaerobic fermentative metabolism and consequently to develop a metabolically engineered strain capable of producing succinic acid without by-product formation.This study deals with the development of purification and separation processes required to produce the highly purified succinic acid from the fermentation broth produced by recombinant microorganism, Mannheimia succiniciproducens. The developed process consists of the pretreatment step of vacuum distillation and the crystallization step for the highly purified succinic acid production. Fermentation broth, separated from cells in the fermentation broth by the centrifugation, is applied to the pretreatment processes which is the vacuum distillation for concentration and removal of the volatile contaminated organic acid. The crystallization of highly purified succinic acid from the pretreatment sample solution is conducted at adjusting the pH.