Influenza virus capture using membrane chromatography: Improving selectivity by matrix design and pseudo-affinity ligand interactions

摘要

Membrane chromatography is consistently used in the purification of viral particles like adenoviruses or influenza viruses. The lack of traditional diffusion-based limitations of porous particles and increased binding capacities in a disposable format make it a viable alternative to bead chromatography. This poster presents a novel cellulose based stationary membrane whose specific surface area is designed for maximum virus accessibility. The membrane also utilizes highly selective pseudo-affinity ligands for influenza viruses resulting in an overall increase in selectivity and product recovery. The unique capabilities of this media not only contribute to reduction of the costs associated wilh the bind Et clutc purification of viruses but may also constitute one step forward in the development of an optimized and efficacious purification platform process for the vaccine industry.