Dependence of the Energy of Electric Pulse Required to Electroporate the Cell on the Pulse Duration for Pulses from 50 ns to 500 ms

摘要

The aim of this study was to investigate the relationships between the energy of the PEF treatment required to electroporate the cell and pulse duration and to determine whether there exists the electric pulse with the minimum energy required to electroporate the cell. Experiments were performed in vitro with human erythrocytes and mouse hepatoma cell line MH-22A. Cell electroporation was determined from the extent of the release of intra-cellular K~+ and from the extent of erythrocyte lysis after long incubation at low temperature. Energy of an electric pulse required to electroporate the cell was a function of the pulse duration and there existed a minimum of the pulse energy required to porate the cell for both theoretical and experimental dependencies. It was situated in the range of 0.1-10 μs. It depended on the cell size and was about 0.13 and 0.07 J/g for human erythrocytes (radius 3.0 μm) and mouse hepatoma MH-22A cells (radius 7.7 μm), respectively.