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Bioluminescent indicator applicable to membrane voltage recording in various excitable cell types

机译：生物发光指示剂，适用于各种可激发细胞类型的膜电压记录

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摘要

Here, we report a world-first bioluminescent indicator for membrane voltage, LOTUS-V. Since it is able to reveal voltage dynamics without external light source, LOTUS-V serves high contrast voltage imaging free from the effect of autofluorescence, suggesting its great versatility in the wide range of bioscience.

机译：在这里，我们报告了世界上第一个膜电压LOTUS-V生物发光指示器。由于无需外部光源即可显示电压动态，因此LOTUS-V可提供高对比度电压成像，而不受自发荧光的影响，这表明LOTUS-V在广泛的生物科学领域具有广泛的通用性。

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来源
《Biomedical imaging and sensing conference》|2017年|1025102.1-1025102.2|共2页
会议地点 Yokohama(JP)
作者
Shigenori Inagaki; Takeharu Nagai;
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作者单位

Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan;

Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan,The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan;

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原文格式 PDF
正文语种 eng
中图分类
关键词
bioluminescence imaging; voltage indicator; excitable cells; cardiomyocytes;

机译：生物发光成像；电压指示器可激发细胞心肌细胞;

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1. Cell-Type-Specific Optical Recording of Membrane Voltage Dynamics in Freely Moving Mice [J] . Marshall Jesse D., Li Jin Zhong, Zhang Yanping, Cell . 2016,第6期

机译：自由移动小鼠中膜电压动力学的细胞类型特定光学记录。
2. Characterization of impulse propagation at the microscopic level across geometrically defined expansions of excitable tissue: multiple site optical recording of transmembrane voltage (MSORTV) in patterned growth heart cell cultures. [J] . S Rohr, B M Salzberg The Journal of general physiology . 1994,第2期

机译：跨可激发组织的几何定义的扩展在微观水平上的脉冲传播的表征：图案化生长心脏细胞培养物中跨膜电压（MSORTV）的多位光学记录。
3. Whole-cell recording from honeybee olfactory receptor neurons: ionic currents, membrane excitability and odourant response in developing workerbee and drone. [J] . Laurent S, Masson C, Jakob I The European Journal of Neuroscience . 2002,第7期

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4. Bioluminescent indicator applicable to membrane voltage recording in various excitable cell types [C] . Shigenori Inagaki, Takeharu Nagai Biomedical imaging and sensing conference . 2017

机译：在各种易激的细胞类型中适用于膜电压记录的生物发光指示灯
5. Development of functional in vitro assays for toxicological and pharmacological applications based on high throughput electrophysiological recordings of excitable cells [D] . Varghese, Kucku 2008

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6. Cell-type specific optical recording of membrane voltage dynamics in freely moving mice [O] . Jesse D. Marshall, Jin Zhong Li, Yanping Zhang, -1

机译：自由移动小鼠中膜电压动力学的细胞类型特异性光学记录
7. Bioluminescent indicator applicable to membrane voltage recording in various excitable cell types [O] . Shigenori Inagaki, Takeharu Nagai 2017

机译：在各种易激的细胞类型中适用于膜电压记录的生物发光指示灯
8. Aequorin as a bioluminescent indicator for use in the determination of biomolecules in single cells. Final technical report [R] . Sylvia Daunert 2000

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Bioluminescent indicator applicable to membrane voltage recording in various excitable cell types

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