HEAT-MASS TRANSFER IN LARGE CELL SUSPENSIONS (CELLS IN A TERNARY CRYOPRESERVATION MEDIUM: WATER-NACL-DMSO) DURING THE FREEZING PROCESS

摘要

In this research, continuum theory (macroscopic enthalpy model) for multi-component phase change was applied to study the freezing process of bulk sample (cell suspension in a flat blood bag), coupled with the investigation of microscopic mass transfer across cell membrane. Numerical simulation results indicated that distributions of temperature and solute concentration inside the sample are not uniform during the cooling process. As a consequence, the degrees of cell dehydration are different at various locations.