Cloning and analysis of several phytohormone-related genes in super hybrid rice parental lines

摘要

Cloning functional genes in super hybrid rice parental lines is of great importance for the super hybrid rice industry. Based on a database search and bioinformatics analysis, several phytohormone-related genes were suucessfully cloned and sequenced. Auxin receptor TIR1 was cloned from Zhu 1S by the RT-PCR method and designated as OsTIR1 (GenBank accession number EU40058). OsTIR1 is 2,219 bp in length and its complete open reading frame (ORF) is 1,764 bp encoding a protein of 587 amino acid residues. OsTIR1 contains both an F-box motif and a leucine-rich repeat domain. An ethylene-responsive element-binding protein gene (accession number EF507537) and partial sequence ABA receptor (Mg-chelatase H subunit, CHLH) gene (accession number EU569725) were also cloned and submitted to GenBank. Further functional analysis will be conducted to acknowledge their importance in the growth and development of hybrid rice.