ISSR analysis of genetic diversity of rice germplasm with long panicles and big grains

摘要

The genetic diversity of 56 specific rice germplasm accessions with long panicles (30.73–46.3 cm) and big grains (30.7–70.63 g) was analyzed using inter simple sequence repeats (ISSR). This rice was derived from the IRRI new plant type strains and then developed by crossing and radiation. A total of 80 pairs of ISSR primers were applied to screen for polymorphism, with nine of them being tested to produce polymorphic bands. The nine pairs of primers generated a total of 70 bands, of which 47 were polymorphic. Percentage polymorphic bands (PPB)was 66.18%; genetic similarity coefficient (GS) varied from 0.368 to 0.962,with an average of 0.665; genetic diversity index (H) was 0.233; the number of alleles (Na) was 1.662; and the effective number of alleles (Ne) amounted to 1.414. Clustering analysis showed 56 accessions that could be classified into four groups with GS of 0.64: the first cluster consisted of nine accessions;the second and third clusters contained six accessions, respectively; and the fourth group had 35 accessions. The results provided the scientific basis for further studies on their molecular mechanism and use in breeding.