Resuscitation of Viable but Nonculturable Vibrio parahaemolyticus

摘要

It has been shown that many bacteria prossesses the ability to enter a viable but nonculturable (VBNC) state after exposure to adverse environmental conditions. It is thought that VBNC state may represent a survival response by bacteria exposed to potentially injurious environmental conditions. The stresses of low nutrient and low temperature have been shown to be the main causes of the induction of VBNC stages in some pathogenic bacteria. The inability to detect VBNC in the environment by employing routine bacteriological methods poses a potential hazard. Long-term survival of noncultuable-yet-viable pathogenic bacteria could have implications for the transmission of disease. It is proposed that sudden transfer of cells to nutrient rich agar at temperatures optimal for enzyme activities initiates imballance in metabolism inducing a near instantaneous production of peroxide. In the absence of phenotypic adaptation the cells are not equipped to detoxify reactive oxygen intermediates and, as a result, a proportion or all of these cells die.