Proteomic Analysis of the Hepatic Effects of Pentachlorophenol (PCP) on Rare Minnows (Gobiocypris rarus)

摘要

Pentachlorophenol (PCP) is a ubiquitous contaminant that has been shown to lead to hepatoxicity and is implicated in the incidence of liver tumors in human. For further understanding of mechanisms of action and identifying the potential protein biomarkers for PCP exposure,2-DE coupled with mass spectrometry has been used to identify proteins differentially expressed in the livers of rare minnow (Gobiocypris rarus) following PCP exposure of 0.5,5,50μg/L. After comparison of the protein profiles from treated and control groups,39 protein spots were found altered in abundance (>2-fold) from male and female PCP-treated groups. Matrix-assisted laser desorption/ionization (MALDI) tandem time-of-flight mass spectrometry (TOF/MS) analysis allowed the unambiguous identification,and 18 protein spots were identified successfully, 12 proteins in females and 6 proteins in males,respectively. These proteins were involved in transport,metabolism,response to oxidalive stress and other biological process. Of these proteins,4 differentially expressed mRNA encoding proteins were quantitative analysis by Quantitative Real-Time PCR.