Nanoparticles and ultrashort lasers in biophotonics

摘要

Nanoparticles have a widespread use in biophotonics especially as biomarkers and sensors. In this talk another aspect of nanoparticles, in particular gold nanoparticles (AuNP), will be addressed. Using ultrashort laser pulses, the near-field scattering of the electric field at the particles allows the confinement of optically delivered energy into the vicinity of the AuNP. Usually these laser pulses are focused by high NA objectives to enable subcellular manipulation with high cell viabilities. If AuNP are used and are attached to a cell membrane, this membrane can be opened transiently by this interaction, being an efficient delivery method for impermeable molecules into the cell.