The Interaction of Protein-Modified Nanodiamond with Bacterial Cells

摘要

The nanometer-sized diamond functionalizing and optimization of this process are studied. To create the functional groups on the nanodiamond surface the carboxylation/oxidization of 100 nm nanodiamonds was applied and its IR spectra were analyzed. The surface functionalizing was followed by conjugating with protein (lysozyme) via physical adsorption. Nanodiamond-protein interaction was analyzed using FTIR spectroscopy. The interaction of lysozyme-nanodiamond conjugates with E. Coli bacteria was observed by analyzing the adsorbed lysozyme antibacterial activity. Nanodiamond-lysozyme conjugates displayed high activity, equivalent to activity of lysozyme in solution. The results demonstrated that the lysozyme preserved its functionality in conjugates with nanodiamond, while in the same time nanodiamonds can serve as probe using spectroscopic methods.