Quantum Dot Nanocrystals in Neuroscience

摘要

Quantum dot nanocrystals are nanometer sized semiconductor particles which can be used for labeling biological systems. They are much brighter and more stable than conventional fluorophores. However, the potential for the use of quantum dots in biological sciences has yet to be realized because of the lack of reliable labeling methods using commercially available reagents. We have developed protocols for labeling neurons and glial cells with commercially available quantum dots, and have used these tools to label glial fibrillary acidic protein (GFAP) intermediate filament cellular bridges. These previously unidentifiable characteristics are now visible with quantum dots, which allow for much higher resolution and more specific labeling than conventional fluorophores.