IMMUNOSENSING USING FABRICATED PROTEIN MICROARRAYS

摘要

Patterned microarrays of antibodies were fabricated and tested for their ability to bind targeted proteins and bacteria. These arrays were used for a series of protein and bacterial immunoassays to detect Immunoglobulin (IgG) proteins, Escherichia coli 0157:H7 and Renibacterium salmoninarum (RS) bacteria. The microarrays were fabricated using microcontact printing (μCP) and microfluidic techniques. Characterization of the microarrays was conducted by scanning probe microscopy (SPM) and fluorescence microscopy (FM). The high-resolution SPM imaging showed that targeted bacteria and proteins had a higher binding selectivity to complementary antibody patterns than to non-functionalized regions of the substrate. These studies demonstrate how protein microarrays could be developed into useful platforms for sensing microorganisms.