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首页> 外文会议>16th International congress on animal hygiene : animal hygiene, health and welfare as corner stones of sustainable animal production >Establishment of the Hybridomas Secreting Monoclonal Antibodies Against Canine Distemper Virus
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Establishment of the Hybridomas Secreting Monoclonal Antibodies Against Canine Distemper Virus

机译：犬瘟热病毒单克隆抗体杂交瘤的建立

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摘要

The splenocyte of BALB/c mice immunized with purified canine distemper virus (CDV)were fused with SP2/0 cells.Twelve hybridomas secreting monoclonal antibodies (mAbs)against CDV,designated as 3H11,ID7,2C9,1F8,G12,F7,H4,A2,2G3,2D9,3G12 and 3G3,were selected with inderect ELISA and cloned by the method of limiting dilution.Monoclonal antibodies secreted from hybridomas 3HII,ID7,2C9,IF8,G12,F7,H4,A2,2G3,2D9,3G12 and 3G3 were identified to be subclass antibodies IgG2bκ,IgG1κ,IgG2bκ,IgG1κ,IgG1κ,IgG1κ,IgG1κ,IgG1κ,IgG1κ,IgG1κ,IgG1κ and IgG1κ.ELISA titers of the acsites were 106-109.Indirect immunofluorescence assay (IFA)showed that the twelve mAbs could combine with the natural CDV specifically.
机译：将纯化的犬瘟热病毒（CDV）免疫的BALB / c小鼠脾细胞与SP2 / 0细胞融合。十二个针对CDV分泌单克隆抗体（mAb）的杂交瘤，命名为3H11，ID7、2C9、1F8，G12，F7，H4分别通过Inderect ELISA选择A2,2G3,2D9,3G12和3G3并通过有限稀释法克隆。杂交瘤3HII，ID7、2C9，IF8，G12，F7，H4，A2、2G3、2D9， 3G12和3G3被鉴定为亚类抗体IgG2bκ，IgG1κ，IgG2bκ，IgG1κ，IgG1κ，IgG1κ，IgG1κ，IgG1κ，IgG1κ，IgG1κ，IgG1κ和IgG1κ亚基的ELISA滴度为106-109。间接免疫荧光法（IFA）显示十二种单抗可以与天然CDV特异性结合。

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  • 会议地点 Nanjing(CN)
  • 作者

    Bi Zhenwei; Wang Yongshan; Xia Xingxia; Sun Jing; Fan Hongjie;

  • 作者单位

    Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology,Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-products,Nanjing 210014,China;

    Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology,Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-products,Nanjing 210014,China;

    Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology,Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-products,Nanjing 210014,China;

    Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology,Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-products,Nanjing 210014,China;

    College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 兽医公共卫生学（家畜卫生学）;
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