STABLE SYSTEM FOR THE IN VITRO CULTURE OF CEREBELLAR GRANULE CELL PRECURSORS (GCP), STABLE METHOD FOR THE IN VITRO CULTURE OF SAID CELLS AND USE OF SAID SYSTEM OR METHOD FOR IN VITRO CULTURE

摘要

This invention relates to an in vitro system for the culture of cerebellar granule cell precursors (granule cell progenitors, GCP) comprising a culture support, mammalian GCP cells and a culture medium comprising at least SAG (Smoothened agonist) and EGF (Epidermal Growth Factor), and a method for the in vitro culture of GCP cells, use of the above-mentioned culture system or method of culture for the generation of in vitro models for study of the pathophysiology of cerebellar granules or use of the above-mentioned culture system or method of culture for use in gene therapy and cell therapy approaches to cerebellar diseases caused by damage or neurodegeneration.