Pattern-Based Detection of Different Proteins Using an Array of Fluorescent Protein Surface Receptors

摘要

There is currently a pressing need for simple, robust, and high throughput strategies for the detection of specific proteins in different environments. For example, large-scale proteomics projects require the facile detection of signaling proteins as they rise and fall as a result of cellular stimulus. Similarly, in medical diagnostics, it is important to determine the presence or absence of the characteristic protein signature of a disease. Finally, current concerns about bioterrorism have raised an immediate need for field detectors that can identify airborn or dissolved pathogens and their toxic protein byproducts. Any protein detectors must be responsive and/or applicable to a wide range of different targets. As a result, there has been extensive development of multiple protein binding molecules in an array format, with the expectation that different proteins will interact with the array in distinctive ways. Effective protein-detecting arrays have been based on monoclonal antibodies, antibody fragments, or nucleic acid aptamers.