Pseudo-CSA Restraints for NMR Refinement of Nucleic Acid Structure

摘要

Residual dipolar couplings(RDCs)provide global orientational restraints that have proven to be particularly useful in NMR structural studies of oligonucleotides,which frequently lack tertiary contacts.One-bond ~(13)C-~1H RDCs can be measured when the macromolecule of interest is weakly aligned relative to the magnetic field,most commonly accomplished by means of a dilute,aqueous lyotropic liquid crystalline medium consisting of bicelles or filamentous bacteriophage,or by use of anisotropically compressed acrylamide gels.