Hybrid Molecular Probe for Nucleic Acid Analysis in Biological Samples

摘要

We have designed and synthesized a hybrid molecular probe for nucleic acid monitoring with high sensitivity and fast hybridization kinetics.This probe has the advantage of no false signals caused by the complex sample matrixes such as living cells.Full deciphering of life processes demands the study of biochemical events within living cells.1 Although current RNA analysis techniques,including in situ hybridization,northern analysis,RT-PCR and microarray technology,have become powerful and indispensable tools in gene expression studies,,they reveal little dynamic information on RNA synthesis,transportation,and localization in living cells.