A Unique Flavin Mononucleotide-Linked Primary Alcohol Oxidase for Glycopeptide A40926 Maturation

摘要

The teicoplanin homologue A40926 differs from teicoplanin (1) mainly in lacking the GlcNAc on residue 6 (r6) and the replacement of the N-acyl glucosaminyl C-6 OH substituent on residue 4 (r4) with a COOH group. We and others have reported that three enzymes, Dbv9, Dbv21, and Dbv8, act sequentially to add the N-acyl glucosaminyl moiety to r4 of teicoplanin or A40926 aglycone (2). The N-acyl glucosaminyl substituent might also undergo further C-6 oxidation to N-acyl aminoglucuronic acid during A40926 biosynthesis, but whether this occurs as the last step in this biosynthesis series has yet to be determined.