Temperature-Regulated Activity of Responsive Polymer-Protein Conjugates Prepared by Grafting-from via RAFT Polymerization

摘要

Covalent modification with synthetic polymers is an effective method to tune the solubility, biocompatibility, stability, and activity of proteins, peptides, and nucleic acids. Bioconjugation with stimuli-responsive polymers is particularly useful because the responsive nature of the polymer can be conferred to the substrate to which it is attached. We are primarily interested in preparing "smart" polymer-protein conjugates to facilitate applications in drug delivery, enzymatic catalysis, biosensing, bioseparation, etc. Realization of these applications requires techniques that streamline synthesis of well-defined responsive polymers and their immobilization onto biomacromolecules.