Origins of the Distortions in the Base Pair Step Adjacent to Platinum Anticancer Drug-DNA Adducts. Fundamental NMR Solution Studies Utilizing Right-Handed Cross-Link Models Having 5'- and 3'-Flanking Residues

摘要

For DNA duplexes, the Lippard laboratory has shown that the XG~* base pair (bp) step has a very unusual slide and shift, where G~* is a G platinated at N7 by di- or monofunctional platinum anticancer drugs. One approach toward understanding the cause of this important unexpected XG~* distortion is to examine single-strand (ss) oligonucleotide (oligo) models. Both duplex and ss XG~*G~* models of the key G~*G~* cross-link formed by cisplatin have the HH1 conformation with head-to-head bases. Cross-links have R canting (3'-G~* H8 atom toward 5'-G~*) in duplexes and L canting (5'-G~* H8 atom toward 3'-G~*) in ss models. However, dynamic motion in solution makes the ss features difficult to define. Thus, we employ less dynamic cross-link models such as (R,S,S,R)-BipPt(d(TG~*G~*)) and (R,S,S,R)-BipPt(d(pG~*G~*TTT)), the first examples of an HH1 conformer with R canting for ss oligos longer than d(GpG) (Bip = 2,2'-bipiperidine). In these, the 5'-T residue decreases R canting (indicating steric clashes with the 5'-G~*) and the less bulky 5'-phosphate group forms a H-bond to HN-Pt (indicating that R canting allows H-bonding). We conclude that the 5'-X residue in duplex adducts changes its position from that in B form DNA to avoid steric clashes with the 5'-G~* and the carrier ligand and secondarily to form a Watson-Crick base pair. These features, possibly aided by weak carrier-ligand H-bonding, lead to the relatively unusual features distinctive to the "Lippard bp step".