Site-specific Immobilization And Micrometer And Nanometer Scale Photopatterning Of Yellow Fluorescent Protein On Glass Surfaces

摘要

The development of methods for molecular patterning with nanos-cale spatial resolution remains a significant challenge in the burgeoning field of bionanotechnology. While there has been a great deal of progress in the development of techniques based on scanning probe microscopy, such as dip-pen nanolithography, nanoshaving/graft-ing, and near-field optical methods, much work has focused on nucleic acids, and there are fewer reports of protein patterning. Moreover, much work has also utilized self-assembled monolayers (SAMs) of alkanethiols on gold as templates for molecular patterning. In addition to a limited stability, they suffer the disadvantage that gold quenches optical activity, rendering the interrogation of biomolecules by spectroscopic techniques more difficult. From the perspective of applications in biology, nanostructures formed on oxide surfaces are particularly attractive, and the oxide of choice would be, in many cases, glass. There have been comparatively few attempts to fabricate nanostructures on silicon dioxide, with the development of constructive nanolithography by Sagiv and co-workers being the most extensive effort to date in this direction.