Toxicity assessment of cadmium chloride on planktonic copepods Centropages ponticus using biochemical markers

摘要

Chemical compounds studied in this article: Cadmium dichloride (PubChem CID: 24947), Acetylthiocholine (PubChem CID: 74629), 5,5′-Dithiobis(2-nitrobenzoic acid) (PubChem CID: 6254), Thiobarbituric acid (PubChem CID: 2723628), Dihydronicotinamide-adenine dinucleotide phosphate (PubChem CID: 5884), Hydrogen peroxide (PubChem CID: 784), Cumene hydroperoxide (PubChem CID: 6629)Keywords: Biomarker, Planktonic copepod, Cadmium, Centropages ponticus

Abstract

Pollution of the aquatic environment by heavy metals has become a worldwide problem. Most heavy metals exhibit toxic waste on aquatic organisms. Cadmium (Cd) is a highly toxic metal which affects aquatic organisms acutely and chronically. Planktonic calanoid copepods are the secondary dominant producers of pelagic ecosystems and play a considerable role in the transfer of energy and organic matter from primary producers to higher trophic levels. We investigated the effect of cadmium chloride on biochemical responses of the planktonic calanoid copepods Centropages ponticus which is a key species in the Mediterranean Sea. The response of copepods to cadmium chloride was examined under laboratory-controlled conditions during a 72-h exposure. Catalase (CAT), Glutathion Reductase (GR), Glutathione Peroxidase (GPx), Glutathione-S-Transferase (GST) and Acetylcholinesterase (AChE) were analyzed for cadmium chloride treatments (0, 0.2 and 0.4 μg/L) after 24, 48 and 72 h. Additionally, the thiobarbituric reactive species assay was used to evaluate lipid peroxidation (LPO) level of the copepod. In this study, it is observed that contents of protein increased gradually with an increase in concentrations of metals and exposure time. Our findings showed that cadmium chloride directly influenced malondialdehyde (MDA) levels in the treated copepods hinting that the copepods had suffered from oxidative damage. During exposure, the Cd treatments significantly influenced the biochemical markers (CAT, GR, GPx, GST and AChE). Thus, Centropages ponticus could be used as a suitable bioindicator of exposure to Cd using biochemicals markers.