Protective role of Emblica officinalis hydro-ethanolic leaf extract in cisplatin induced nephrotoxicity in Rats

摘要

class="kwd-title">Abbreviations: BUN, blood urea nitrogen; BW, body weight; BSA, bovine serum albumin; CAT, catalase; DNA, deoxy-ribonucleic acid; DMSO, dimethyl sulfoxide; DTNB, 5,5-dithio-bis(2-nitrobenzoic acid); FDA, food and drug administration; GPx, glutathione peroxidase; GR, glutathione reductase; GAMT, guanidinoacetate methyltransferase; H&E, hematoxylene and eosin; MDA, malondialdehyde; NaCl, sodium chloride; NADPH, nicotinamide adenine dinucleotide phosphate; RBCs, red blood cells; ROS, reactive oxygen species; RNS, reactive nitrogen species; SOD, superoxide dismutase; SEM, standard error mean; TBA, 2-thiobarbituric acid; TCA, trichloroacetic acid class="kwd-title">Keywords: Emblica officinalis, Cisplatin, Nephrotoxicity, Oxidative stress, Rats class="head no_bottom_margin" id="abs0015title">AbstractNephrotoxicity is a major limiting factor in cisplatin treatment. In the present study hydro-ethanolic leaf extract of Emblica officinalis was investigated for its protective role in cisplatin induced nephrotoxicity. The experiment was designed for 14 days and male Wistar rats were divided into 9 groups (n = 5). Group 1 served as control (with no treatment), group 2 served as a vehicle control and received 0.9% NaCl intraperitoneally (i.p.) on 11th day of the treatment, group 3 received a single dose of cisplatin on 11th day (12 mg/kg body weight, i.p.), group 4–6 received leaf extract only (100 mg/kg, 200 mg/kg and 400 mg/kg body weight, respectively) throughout the treatment, group 7–9 received leaf extract (100 mg/kg, 200 mg/kg and 400 mg/kg body weight, respectively) throughout the treatment and single dose of cisplatin on the 11th day of the leaf extract treatment. At the end of the experiment (i.e. on 14th day) blood samples were collected from all the groups and were sacrificed to study renal functional parameters. Treatment with above doses of E. officinalis leaf extract significantly (p ≤ 0.05) attenuates renal damage by decreasing serum creatinine and blood urea nitrogen (BUN), enhanced the activities of Catalase, SOD, GPx, GR and decreased the renal MDA level compared with the cisplatin treatment group. Furthermore the oral administration of Amla leaf extract improves histological damage and morphological changes in RBCs. Our results suggest that, leaf extract of E. officinalis may ameliorate renal damage caused by cisplatin.