Cellulase was immobilized by chitosan gel beads using the cross-linking embedding method. The immobilized enzyme was used to hydrolyze cellulic material corn stover pretreated by steam explosion , ball milling, ultrasonic, spray, and high-temperature processes. Results showed that the steam explosion pretreatment could obtain the best enzymatic hydrolysis efficiency, glucose yield reached 95% ; the ball mill was second to it, glucose yield reached 60%. Meanwhile, the cellulose structures before and after pretreatment were characterized by electron microscopy and FT-IR. It indicated that the physical structure and chemical composition of pretreated materials changed significantly. The steam explosion method and the ball milling method could make the natural structure of the material completely destroy thus increasing the surface area. The internal hydrogen bonds and functional groups of cellulose had been changed during the course of steam explosion, it caused the material more easily to be hydrolized.%以壳聚糖为载体用交联法制备固定化纤维素酶,考察固定化纤维素酶对蒸爆、球磨、超声波、喷淋、高温预处理玉米秸秆纤维素原料的酶解效果.结果表明:物料经蒸爆预处理后酶水解效率最高可以达到95%,球磨预处理水解效率次之,达到60%.用电镜和FT-IR对处理前后秸秆结构进行表征分析,证明预处理对物料的物理结构及化学组成有一定的影响.蒸爆法和球磨法可以使物料致密的天然结构彻底破坏,从而增加物料的比表面积;蒸爆预处理可以使纤维素内部氢键和官能团改变,使物料更易于酶解.
展开▼
