Induced miR-30e-5p expression represses YAP1 cooperatively with miR- 15a to promote fat metabolism

摘要

Fat metabolism is a complicated process regulated by a series of factors.microRNAs (miRNAs) is a class of 18 ～25 nt small RNAs that negatively regulate gene expression and play crucial roles in many biological processes,including fat metabolism.Here we utilize an improved miRNA detection assay,S-Poly(T) to profile the expression of miRNAs in the goat mammary gland of peak-lactation and dry-lactation and goat mammary gland epithelial cells (GMEC) of prolactin treatment,finding that miR-30e-5p and miR-15a expresses abundantly in goat mammary gland and prolactin treatment.Moreover,we demonstrated that the over expression of miR-30e-5p and miR-15a promoted fat metabolism while the knockdown of miR-30e-5p and miR-15a impaired fat metabolism in GMEC,These findings extend the discovery of miR-30e-5p and miR-15a functioning in mediating adipocyte differentiation,by suggesting its role in promoting fat metabolism,which develops our understanding about the significance of miRNAs in milk fat synthesis.In this study,we find that over expressed miR- 30e-5p and miR-15a promoted adipogenesis and inhibited miR-30e-5p and miR-15a impaired adipogenesis in goat mammary epithelial cells.Utilized 3'-UTR assay and Western Blot,LRP6,important genes of Wnt path ways and YAP1,important genes of hippo pathways,was demonstrated to be a potential target of miR-30e-5p and miR-15a.We reveal the miR-30e-5p cooperation with miR-15a regulation YAP1 promote adipogenesis.Interestingly,we also verified miR-30e-5p can regulation β-catenin,β-catenin can regulation YAP1and miR-30e-5p expression represses YAP1 cooperatively with miR-15a during fat metabolism in GMECs.In conclusion,our findings indicate that miR-30e-5p cooperate with miR-15a promote fat metabolism by repressing LRP6 and YAP1 expression and this contributes to further understanding about the functional significance of miRNAs in milk fat synthesis.